Specifications SUPREMA columns
|
Applicability |
Neutral and anionic polymers, (PEO, PEG, Pullulan, Dextran, Poly(acrylamide), Hyaluronic acid, Poly(acrylic acid), Carboxymethylcellulose, etc.) |
|
Eluents |
water (with salts/buffers, MeOH, ACN) pH: 1.5 – 13 |
|
Material |
Polyhydroxymethacrylate copolymer network |
|
Maximum Pressure |
50 - 80 bar (725 - 1160 psi), depending on porosity |
|
Maximum Temperature |
80° C |
|
Maximum Flow Rate |
2 ml/min (I.D.: 8 mm) |
|
Particle Size |
10 and 20 µm |
|
Molecular Weight Range |
100 to > 30 000 000 Da |
|
Incompatibility |
Drying and freezing |
|
Recommended Column Combination |
Low molar mass: 100 Å + 100 Å |
Separation range
|
30 Å |
100 |
- |
30 000 Da |
|
100 Å |
100 |
- |
100 000 Da |
|
1 000 Å |
100 |
- |
1 000 000 Da |
|
3 000 Å |
1 000 |
- |
3 000 000 Da |
|
10 000 Å |
1 000 |
- |
10 000 000 Da |
|
30 000 Å |
5 000 |
- |
> 30 000 000 Da |
|
linear S |
100 |
- |
100 000 Da |
|
linear M |
1 000 |
- |
1 000 000 Da |
|
linear XL |
5 000 |
- |
3 000 000 Da |
Calibration curves: single porosity columns
Calibration curves: linear columns
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