Specifications PROTEEMA columns
|
Applicability |
Natural and synthetic Proteins, Peptides, Enzymes, Gelatins/Collagens |
|
Eluents |
Water, water with salt/buffer; pH: 2 -9 |
|
Material |
Special modified silica |
|
Maximum Pressure |
150 - 200 bar (2180 - 2900 psi), depending on porosity |
|
Maximum Temperature |
70° C |
|
Maximum Flow Rate |
(8 mm I.D.) 3 ml/min |
|
Particle Size |
5 µm |
|
Molecular Weight Range |
100 to 7 500 000 Da |
|
Incompatibility |
drying, and freezing |
|
Recommended Column Combination |
Low molar mass: 100 Å + 100 Å |
Separation range*
|
100 Å |
100 |
- |
150 000 Da |
|
300 Å |
1 000 |
- |
1 200 000 Da |
|
1 000 Å |
1 000 |
- |
7 500 000 Da |
* based on protein molecular weight, for other water soluble macromolecules
(e.g. pullulane) separation range ends at 50 000, 400 000, and 2 500 000 Da.
Calibration curves: single porosity columns
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