GPC/SEC HighSpeed Columns
The PSS HighSpeed family of columns permits dramatic reductions in GPC analysis time, for polymers > 2000 Da without loss in resolution, accuracy or reproducibility. PSS HighSpeed columns have a larger diameter and a shorter length (20mm x 50mm) than their typical analytical counterparts (8mm x 300mm), and the ability to operate at optimum flow rates of 6.25 ml/min while keeping same linear flow, equal resolution and polymer information: Mw, Mn, Mp and PDI.
HigSpeed columns have unparalleled ability to produce a 12 point calibration curve in less than ten minutes; (Polystyrene 374 to 2 570 000 Da shown below). Optimum column design helps expedite product screening, process control,
and rapid sample throughput.
These columns can substitute the analytical columns without modifications to your LC system, as long as your pump and detectors can accommodate 6.25 ml/min. At this flow rate, you will use the same amount of solvent you currently use, but your elution occurs quicker.
HighSpeed columns are available with different column materials, particle sizes, and porosities, as linear and as single porosity columns.
Calibration with Poly(styrene) ReadyCals green, red, and white: cocktails of 4 different molecular weight standards
Conditions
| Column | PSS SDV 5µ linear M, HighSpeed column |
| Stationary phase | adjusted for high pore volume and resolution |
| Flow rate, Time consumption | 6.25 ml/min, 7 minutes for THREE SAMPLES |
| Eluent, Eluent consumption | THF, 44 ml for THREE SAMPLES |
| Temperature | 25 °C |
| Detection | GPC1100 RI |
| Samples | PSS Poly(styrene) ReadyCal green, red, white |
| Injection interval | 2.1 minutes (13.5 ml, overlaid injection not used) |
| Injection volume | 30 µL |
Application example
A HighSpeed column (red line) reduces the run time for a sample (broad Poly(styrene)) by a factor of 6 - 8 compared to a conventional column (black line).