GPC/SEC column types and applications
1: analytical column 8 x 600 mm
2: preparative column 8 x 300 mm + precolumn
3: analytical column 8 x 300 mm + precolumn
|
Column type |
Column dimension |
Field of application |
|
Precolumn |
|
Protection of separation columns |
|
Analytical |
8 x 300 | |
|
Micro |
4.6 x 250 |
GPC/SEC analysis optimized for solvent saving and small sample amounts |
|
Preparative |
20 x 300 |
Preparative sample fractionation, sample collection with GPC/SEC |
|
20 x 50 | ||
|
8 x 300 |
Optimized for GPC/SEC-Light Scattering |
* I.D.: internal diameter
** Any other column dimension on request
Column particle size
PSS GPC/SEC column materials are available in several particle sizes from 3 μm up to 20 μm particles.
The optimum particle size for a given application depends on
- the solvent viscosity:
higher viscosity requires higher particle size - the sample molar mass:
higher molar masses require higher particle sizes.
Porosity
The pore size distribution of the column material determines the separation range.
- To separate low molecular weight products use small porosities.
- The higher the molecular weight the higher is the required porosity.
Single porosity columns show a high resolution in a narrow molecular weight range. A wide molecular weight range at constant high resolution is covered if columns with single porosities are coupled in series (column combination).
Examples for single porosity columns:
- SDV 1 000 Å, separation range: 100 - 60 000 Da
- GRAM 3 000 Å, separation range: 5 000 - 5 000 000 Da
- SUPREMA 1 000 Å, separation range: 100 - 1 000 000 Da
Examples for recommended column combinations (series of single porosity columns):
- SDV 100 Å + 1 000 Å + 10 000Å + precolumn (low molar masses)
- GRAM 30 Å + 1 000 Å + 1 000Å + precolumn (medium molar masses)
- SUPREMA 100 Å + 3 000 Å + 3 000Å + precolumn (high molar masses)
Linear or mixed bed columns provide a very wide porosity range therefore offering a broad separation range with a smaller resolution.
Examples for linear columns:
- SDV linear M separation range: 100 - 1 000 000 Da
- GRAM linear, separation range: 500 - 1 000 000 Da
- SUPREMA linear XL, separation range: 1 000 - 4 000 000 Da
Advantages column combination: high resolution" rel="fancybox401" class="jqfancybox">
Advantages linear column: fast analysis time, broad molar mass range with one column
Advantages column combination: high resolution
PSS offers the optimum column or column combination for any application.
See the
- overview with the column specifications to decide what solution is best for you,
- Search our (column) application database,
- request our consumables catalog with all recommendations or
- ask for a column selection.