Column selection guide
GPC/SEC columns can be selected by different criteria depending on your analytical goal (highest resolution, product screening, etc.). This page will present the most important and essential criteria.
1.Step: Optimum Stationary Phase
The PSS column concept is to minimize interactions between polymer and stationary phases by providing stationary phases of different polarities.
In general, the sample is the determinant factor: the polarity of the sample defines the polarity of the solvent and therefore also that of the stationary phase. To ensure interaction free chromatography the stationary phase needs to be selected carefully.
The PSS Magic Triangle provides a quick visual aid for the selection of columns, where the three sides represent sample, mobile phase, and stationary phase.
Handling of the PSS Magic Triangle
Construct an isosceles triangle by locating the polarity of sample and mobile phase; the peak of the Magic Triangle guides you to the proper stationary phase. This concept is also applicable for aqueous GPC/SEC. The magic triangle for aqueous systems takes the pH value instead of the stationary phase into account.
If in doubt...
use the PSS column application database.
Our Search allows to search for products, column applications, publications and software applications.
If your application is not there contact us or your closest representative for further information.
PSS offers also a column selection service for new applications.
2. Step: Field of Application
|
Select |
for |
|
Analytical linear or mixed bed GPC/SEC columns |
Product screening at constant peak resolution |
|
Combination of analytical single porosity GPC/SEC columns |
Highest resolution and maximum information Use the column combinations recommended by PSS to avoid column mismatch. |
|
Very fast results needed in process control and high throughput screening | |
|
Micro GPC/SEC columns |
Solvent saving, small sample amounts |
|
Preparative GPC/SEC columns |
Preparative sample fractionation, sample collection |
|
GPC/SEC-light scattering or Triple detections |
In any case use GPC/SEC precolumns for the protection of the main columns.
Tip: For additional column dimensions please call us or your closest representative.
3. Step: Particle Sizes and Porosities
Select the proper particle size and porosities.
See column specifications for the availability and the molar mass range.